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Effect of spermidine (Spd) and cycloheximide (Cyh) additions on the ornithine decarboxylase activity of a polyamine-deficient spe2–4 mutant (strain 28-8D) that is derepressed for ornithine decarboxylase. At 0 time, cycloheximide (final concentration 200 μg/ml) was added to the incubations labeled “+Cyh.” At 30 min, spermidine (final concentration 100 μM) was added to the incubations labeled “+Spd” (solid lines).
