Table 1.
Strains
| S. cerevisiae strain | Characteristics | Source or reference |
|---|---|---|
| BJ1991 | prb1-1122 pep 4-3 leu2 trp1 | E. W. Jones |
| SPE1SPE2 | ||
| 28-8D | SPE1 spe2-4 | American type culture collection no. 42295, ref. 17 |
| Y362 | ura3-52 leu2Δspe1∷LEU2 Δspe2∷LEU2 | Ref. 18 |
| Y387 | prb1-1122 pep 4-3 leu2 trp1 | BJ1991/pVT101U/SPE1 |
| SPE1SPE2 pVT101U/SPE1 | ||
| Y465* | ura3-52 leu2Δspe1∷LEU2 | Y362/pVT101U/SPE1 |
| Δspe2∷LEU2/pVT101U/SPE1 |
*
This construct is similar to that described by Balasundaram, et al. (19), except for different auxotrophic markers in the host strain (Y450) used in the latter paper. Y362 is ura3-52 leu2 Δspe1∷LEU2 Δspe2∷LEU2, while Y450 is arg4 ura3-52 trp1-289 thr1 leu2 Δspe1∷LEU2 Δspe2∷LEU2.