Table 3.
Specific activity* | |
---|---|
No spermidine addition | 3,200 |
1.5 hr after spermidine addition | 1,900 |
3.3 hr after spermidine addition | 1,900 |
nmol CO2 formed/hr/mg protein.
Y465 was grown in SD medium containing 10−9 M spermidine, as described in Materials and Methods. The doubling time in 10−9 M spermidine was approximately 5 hr. At zero time, spermidine was added to a final concentration of 10−4 M. Only a small increase in optical density was observed during the 3.3 hr after the spermidine addition.