Fig 1. Gene and protein expression of plasma membrane and intracellular localized TLRs and of the IFN regulatory factors IRF3 and IRF7 in murine and human cardiac tissue under basal conditions.

Cardiac tissue of healthy C57BL/6J wildtype mice was used for TaqMan based gene expression analysis of various TLRs and their IFN regulatory factors Irf3 and Irf7 under basal conditions. Gene expressions of Tlr4, Tlr9, Irf3 and Irf7 were highly expressed when compared to the remaining TLRs under basal conditions in murine cardiac tissue. The highest gene expression under basal condition was detected for Irf7. In addition, we detected similar human protein expression patterns when compared with murine gene expression in cardiac tissue under basal conditions. Data are presented in box plots as absolute mRNA expression normalized to the house keeping gene Cdkn1b and human protein abundance are presented as rhombus sign on the right-hand of each mRNA expression.