Table 1. Sequences of the primers used for RACE, full-length amplification, real time PCR and vector construction.
| Gene | Methods used | Primers (5’-3’) |
|---|---|---|
| DkNAC7 | 3’RACE (Primary PCR) | CAAGCCCTTCCGATTCGATGCCAT |
| DkNAC7 | 3’RACE (Secondary PCR) | GGAAGACAACAGGAAAGGACAGGCC |
| DkNAC7 | 5’RACE (Primary PCR) | CTCGTCATCCTCCCATTCCTCCTCAAC |
| DkNAC7 | 5’RACE (Secondary PCR) | CTCGTGCATCACCCAGTTGGTCCTCT |
| DkNAC7 | Full-length clone (FP) | CATCGGCGGTGACCAAAACGG |
| DkNAC7 | Full-length clone (RP) | CACAAAGTCCCTAGATCTCAGA |
| DkNAC7 | Y1H constructs (FP) | CGCGAATTCATGGGCCTCGATCCATCGTC |
| DkNAC7 | Y1H constructs (RP) | GATGGATCCCTACCTCGATGCATTTCCCG |
| DkNAC7 | SK vector construction (FP) | CGCGCGGCCGCATGGGCCTCGATCCATCGTC |
| DkNAC7 | SK vector construction (RP) | GATGGATCCCTACCTCGATGCATTTCCCG |
| DkNAC7 | Q-PCR (FP) | TGAGTTTCAAAATTGGGAGT |
| DkNAC7 | Q-PCR (RP) | CCCTAGATCTCAGATGGTGA |
| DkNAC7 | GFP vector construction (FP) | CGCGGTACCATGGGCCTCGATCCATCGTC |
| DkNAC7 | GFP vector construction (RP) | CATGTCGACCCTCGATGCATTTCCCG |
| DkERF9 | pAbAi vector construction (FP) | CGCGAGCTCAAATAATTTAATTAAAGATA |
| DkERF9 | pAbAi vector construction (RP) | CGCGTCGACATACACAGGAAAACAGGATT |
Note: underlined sequences show cutting sites for restriction enzymes