Fig 4. RVC does not affect tissue morphology or the expression and distribution of proteins in tissue samples.

(A) Morphological features of murine liver tissue samples stored in different preservative solutions. (B) The abovementioned paraffin-embedded tissue samples were sliced and stained with H&E for the examination of cellular morphological characteristics. RNAlater and 100% ethanol caused significant tissue dehydration, whereas the RVC-based preservative solution did not cause this phenomenon. (C-D) Determination of EGFR and PCNA protein expression and distribution by immunohistochemical staining in tissue samples treated with different preservative solutions. The results from the RVC-based groups were consistent with the data of the control group.