Table 3. Genetic diversity and neutrality tests for total samples and for geographic and haplogroups of Protonectarina sylveirae (according to the haplotype network).

		Genetic Diversity					Neutrality Tests*
Sample	N	H (no. in network)	Hd	Π	S	K	Tajima’s D	Fu’s Fs	R2
Total	117	35 (1–35)	0,921±0,015	0,01445±0,00078	144	33,496	-0,08987	-0,70984	0,08917
A	24	9 (2, 3, 24–29, 33)	0,812±0,058	0,00417±0,00028	27	9,66304	-0,06530	0,03461	0,12437
B	10	2 (22, 23)	0,356±0,159	0,00077±0,00034	5	1,77778	-0,08276	0,21385	0,19208
C/CE	67	15 (1, 4–15, 20, 21)	0,802±0,037	0,00430±0,00029	38	9,9638	-0,10060	-0,26695	0,09959
D	10	3 (30–32)	0,600±0,131	0,00029±0,00008	2	0,66667	-0,03511	0,24600	0,22369
E	4	4 (16–19)	1,000±0,177	0,01661±0,00736	74	38,5000	-0,01890	2,02953	0,24360
F	2	2 (34, 35)	1,000±0,500	0,00086±0,00043	2	2,0000	—	0,81599	0,31076
NE	16	9 (16–19, 30–32, 34, 35)	0,850±0,077	0,01275±0,00299	103	29,5583	-0,11091	0,17213	0,13588
SO	34	11 (2, 3, 22–29, 33)	0,856±0,034	0,00876±0,00066	56	20,3226	-0,03782	-0,24629	0,11423

* Average number.

N: number of samples; H: number of haplotypes; Hd: haplotype diversity; π: nucleotide diversity; S: number of variable sites; k: average number of nucleotide differences; ±: standard deviation. Values of p-value: R2: 0,000; Tajima’s D and Fu’s Fs: not significant. Groups shown in Fig 4.