Table 1.
Fitted half-times and amplitudes of TyrZ • exponential decay after induction by 532 and 732 nm laser flash in the Tris-washed PSII membranes without any additions or in the presence of 2 mM ferricyanide (Ferri) and 2 mM ferricyanide and 1 mM DPC (Ferri + DPC)
| t1/2, ms (Ampl., %) | pH 4.7 | pH 6.3 | pH 8.5 | ||||||
|---|---|---|---|---|---|---|---|---|---|
| No add. | Ferri | Ferri + DPC | No add. | Ferri | Ferri + DPC | No add. | Ferri | Ferri + DPC | |
| 532 nm | 22 ± 2 ms (34%) | 22 ± 2 ms (85%) | 10 ± 1.5 ms (42%) | 222 ± 25 ms (43%) | 118 ± 15 ms (95%) | 17 ± 2 ms (55%) | 557 ± 53 ms (48%) | 436 ± 49 ms (109%) | 59 ± 7 ms (25%) |
| 732 nm | 21 ± 2 ms (18%) | 24 ± 2 ms (20%) | 6 ± 1 ms (8%) | 170 ± 21 ms (19%) | 102 ± 13 ms (27%) | 24 ± 2 ms (25%) | 596 ± 53 ms (20%) | 338 ± 37 ms (37%) | 76 ± 7 ms (16%) |
The standard error in the signal amplitude estimation in our EPR measurements was <5%