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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Bioorg Med Chem Lett. 2018 Feb 14;28(6):1024–1029. doi: 10.1016/j.bmcl.2018.02.027

Fig. 4.

Fig. 4

Characterization of initial compounds 2–3. A) Chemical structures and KD values of compounds 2–3 in the fluorescence quenching assays. B) STD-NMR experiment results: 1H spectra (black) and the STD spectrum (blue) of compound 3. Note that the 1H NMR of compound 3 indicated a mixture of two rotamers with estimation of 1:1 ratio. One of the two rotamers binds favorably to apo pa-HemO. C) 1H,15N-HSQC NMR results of compound 3. Black: apo pa-HemO; Red: compound 3 mixed with apo pa-HemO.