Figure 4.

Relative proximity ratio E_sm* as a function of the voltage applied for design A₁ (A) and for design A₂ (B) from capillary experiments and coarse-grained simulations. E_sm* is indicated relative to the value in the absence of an applied voltage i.e. E*(x mV) – E*(0 mV). For the unscaled proximity ratios, see Supplementary Figure S8. We used N_tot = 185 traces from 8 capillaries in design A₁ and N_tot = 241 traces from 6 capillaries in design A₂. Experimental zero voltage values were obtained by measuring the I_A(D*) signal from origami structures immobilized on a coverslip using a BSA-biotin-neutravidin coating (outlined in Methods). The error bars correspond to the standard error of the mean. The simulated proximity ratio was calculated from the simulated distance between dye labeling sites, r using the expression relating FRET efficiency to distance E* = 1/⟨1 + (r/R₀)⁶⟩ where R₀ = 5.9 nm is the Förster radius.