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. 2018 Feb 12;596(6):1019–1033. doi: 10.1113/JP274524

Figure 6. Functional knockdown or overexpression of BKCa channel γ1‐subunits in UAs isolated from pregnant or non‐pregnant mice.

Figure 6

Basal lumen diameters and 1–100 nm IbTX responses of pressurized UAs from P14 (A and B), P18 (C–F) and NP (G and H) mice transfected with either Scr (grey symbols, A–F), empty vector (grey symbols, G and H), γ1‐subunit‐targeted shRNA (filled symbols, A–D), BKCa‐targeted shRNA (filled symbols, E and F) or γ1‐subunit overexpression construct (filled symbols, G and H). Grey dashed lines represent median diameter values of non‐permeabilized UAs, as shown in Fig. 3 A. Symbols in A, C, E and G are individual values, bars are median values. Symbols in B, D, F and H are mean ± SEM. * P < 0.05 compared to Scr shRNA or empty vector at same IbTX concentration, § P < 0.05 between curves. P14 (B, n = 3–5), P18 (D and F, n = 6–8) and NP (H, n = 8).