Fig. 4.

Knocking down miR-15a expression suppresses NSCLC cell apoptosis. At 24 h after transfection, the cells were cultured with serum-free media for 24 h to induce apoptosis. a. Apoptotic cells were stained with PI and Annexin-V and analyzed by flow cytometry. b. Apoptotic proteins were detected by Western blot analysis, and the results are presented with a histogram. Data are presented as mean ± SEM of three independent experiments, ^*P < .01.