Fig. 4.

T7 terminator library. (A) Synthetic overlapping oligos was annealed together and assembled into the SpeI and NheI digested pETM6-eGFP vector backbone. Box region shows the overlapping region and sequence flanked by SpeI and NheI is the core of the degenerate T7 terminator library. (B) Translational strength of terminator library screened by green fluorescence protein. (C) Statistical distribution of terminator strength across the engineered terminator library.