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. 2017 May 3;70(2):503–512. doi: 10.1007/s10616-017-0101-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Myoblasts seeded on Cytodex^® 1 microcarriers, with new beads added on day 3 or day 7. a MyoD and Myogenin RNA expression by RT-QPCR. b The cells are trypsinized and then seeded on a plate in differentiation medium for 4 days to check myotube formation. Note the elongated structures that represent merged myoblasts. Scale bar = 1000 µm. “prol. Ctrl” is a 2D culture condition optimized for cell proliferation and “diff Ctrl” is optimized for myocyte differentiation. The arrows point to the myotubes