Fig. 1.

miR-889 is a negative regulator of MICB. a Bioinformatic analysis based on Targetscan software suggested a putative miR-889 target site in the 3′-UTR of MICB mRNA. b Luciferase reporter assay. 293T cells were co-transfected with MICB 3′-UTR reporters together with miR-889-expressing plasmid or empty vector and luciferase activities were measured at 24 h after transfection. c qRT-PCR analysis of miR-889 and MICB mRNA in HCC cells transfected with miR-889-expressing plasmid or empty vector. d Western blot analysis of MICB protein in HCC cells transfected with miR-889-expressing plasmid or empty vector. Top representative blots. Bottom densitometric analysis of MICB protein levels. Data are representative of three independent experiments. *P < 0.05 versus cells transfected with empty vector