Fig. 3.

miR-889 attenuates NK cell-mediated cytotoxicity by targeting MICB. a NK cell-mediated cytotoxicity assay. HCC cells were transfected with miR-889-expressing plasmid or together with empty vector or MICB-expressing plasmid and incubated with NK-92 cells at indicated effector to target cell ratios. NK lysis was assessed by measuring LDH release. Data are representative of three independent experiments. b Western blot analysis of MICB protein in HCC cells transfected with indicated constructs. Top representative blots of three independent experiments. Bottom densitometric analysis of Western blots. c HCC cells transfected with anti-miR-889 inhibitors or control inhibitors were incubated with NK-92 cells at indicated effector to target cell ratios and subjected to NK cell-mediated cytotoxicity assay. d Measurement of MICB protein in HCC cells transfected with anti-miR-889 inhibitors or control inhibitors by Western blot analysis. Top representative blots of three independent experiments. Bottom densitometric analysis of Western blots. *P < 0.05