Fig. 1.

Characterization of diaphragmatic myoblasts. a Isolated diaphragmatic myoblasts cultured on a feeder layer of autologous macrophages. b–d Green fluorescence immunostaining for desmin (b), sarcomeric α-actin (c) and SERCA 2 ATPase (d) in cultured diaphragmatic myoblasts. Nuclei are stained blue (DAPI). e Arrow heads show diaphragmatic myoblasts differentiated into myotubes. f, g Green fluorescence immunostaining for MyoD (f) and skeletal muscle myosin heavy chain (g) in cultured myotubes. Bars: 50 μm