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Derivation and cultivation of mouse FGSCs. a The expression and localization of Oct4 in ovarian surface epithelium and cortical surface in OG2 mice were measured via immunohistochemistry. Scale bars, 100 μm. b Isolated cells proliferated with a beaded-like structure in vitro, from left to right, day 2, day 3, day 4. c Despite several passages, the clones still stably expressed the GFP protein under the control of the Oct4 promoter. BF, Bright Field.
