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. 2018 Mar 14;9(3):405. doi: 10.1038/s41419-018-0439-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a-c HepG2 cells and primary hepatocytes that originated from male HFD-fed C57BL/6J WT mice were infected with adenovirus-inhibiting miR199a-5p (Ad-anti-miR199a-5p) or the negative control (Ad-anti-miR NC) for 48 h. d-f HepG2 cells and primary hepatocytes that originated from male C57BL/6J WT mice were infected with adenovirus-expressing miR199a-5p (Ad-miR199a-5p) or the negative control (Ad-miR NC) for 48 h. The expression level of miR199a-5p and the protein levels of ATG14, BECLIN1 and LC3 in HepG2 cells and primary hepatocytes are shown in a, b and d, e, respectively. Primary hepatocytes were co-infected with adenoviruses to express mCherry and GFP double-tagged LC3, and the formation of autophagosomes (yellow) and autolysosomes (red) during the autophagy process were analyzed by fluorescence microscopy (c, f, scale bar: 20 µm, the orange arrows indicate the yellow dots). The two-tailed Student's t-test was applied to determine the difference between the Ad-anti-miR199a-5p group or the Ad-miR199a-5p group and the corresponding control group (**P < 0.01, ***P < 0.001)