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. 2018 Mar 14;9(3):405. doi: 10.1038/s41419-018-0439-7

Fig. 6. miR199a-5p leads to insulin resistance via ATG14 in vitro.

Fig. 6

a HepG2 cells and primary hepatocytes were infected with adenovirus-expressing miR199a-5p (Ad-anti-miR199a-5p) or the negative control (Ad-anti-miR NC) for 48 h. b HepG2 cells and primary hepatocytes were infected with adenovirus-expressing miR199a-5p (Ad-miR199a-5p) or the negative control (Ad-miR NC) for 48 h. c Primary hepatocytes were infected with adenovirus-expressing ATG14 (Ad-ATG14) or the negative control (Ad-NC) for 48 h. d Primary hepatocytes were infected with adenoviruses expressing miR199a-5p (Ad-miR199a-5p) and ATG14 (Ad-ATG14) or the negative control (Ad-NC) for 48 h, and then treated with 5 μM 3-MA for 2 h. All cases were followed with or without 100 nmol/L insulin (Ins) stimulation for 20 min. p-IRβ (tyr1162/1163), p-AKT (ser473) and p-GSK3β (ser9) protein levels and the total protein level were examined by western blot. Data shown are representative of three independent experiments