Fig. 4. Role of AMPK in A77 1726-induced autophagy.

NSC34 cells were incubated in the absence or presence of A77 1726 (200 μM), oligomycin (5 μM) or metformin (10 mM) for 16 h (a, b) or were incubated in the absence or presence of A77 1726 (200 μM) and/or compound C (5 μM) for 16 h (c, d). Cell lysates were prepared and analyzed for total and phosphorylated proteins by western blot. The expression levels were analyzed by quantification of the density of the protein bands with NIH Image-J software and presented as bar graphs (b, d). *p < 0.05; **p < 0.01