Fig. 6. A77 1726 blocks the formation of SOD1^G93A protein aggregates.

a, b NSC34 cells transiently transfected with SOD1-GFP or SOD1^G93A-GFP expression vectors were treated as described in “Materials and Methods” section. The cells were examined under a confocal microscope for SOD1-GFP or SOD1^G93A-GFP expression (a, b) and quantified for the fluorescence intensity in a plate reader (c). The results represent the mean ± SD from one experiment in triplicate. The experiments were repeated twice with similar results. d The anti-proliferative effect of A77 1726 on NSC34 cells. Untransfected NSC34 cells or the cells transfected with the SOD1-GFP or SOD1^G93A-GFP expression vector were seeded in a 96-well plate (5000 cells per well) and incubated in the absence or presence of A77 1726 (200 μM) or rapamycin (50 nM) for 24 h. Cell proliferation was analyzed by an ATP-based Cell-Glo assay. The data are the mean ± SD of the triplicate from one representative of two experiments with similar results. *p < 0.05; **p < 0.01