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. 2018 Mar 14;9:1066. doi: 10.1038/s41467-018-03359-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Immunofluorescence staining of archival brain tissues. a Upper panel: comparison of formalin-fixed human cerebellar tissue immersed in PBS (left), OPTIClear (middle) and OPTIClear after 5 days of SDS delipidation at 55 °C (right). Lower panel: comparison of 5 mm-thick, formalin-fixed human striatum tissue block after 3.5 months of SDS delipidation at 55 °C, before (left) and (after) OPTIClearing. b Colour depth-coded, Z-stack image of a block of 2 mm-thick cingulate cortex that has been formalin-fixed for 50 years, stained with antibodies against GFAP with an imaging depth of 125.57 µm. The tissue was cleared after SDS treatment for 4 months at 55 °C and cleared in OPTIClear. Scale bar = 50 µm. c Z-stack image of a piece of formalin-fixed, paraffin-embedded midbrain tissue immunostained for GFAP (red) and counterstained with DAPI (blue) after dewaxing and rehydration (Z-stack depth = 20.77 µm)