Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 14;8:4533. doi: 10.1038/s41598-018-22668-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Recovery rate of D1 receptors as a function of GCA. Animals from the high and low GCA samples (see Fig. 1) were administered EEDQ (8 mg/kg), which irreversibly binds the D1 receptor. Receptors that can be detected after EEDQ injection are indicative of newly available receptors. Control animals received vehicle injections and served as a baseline from which to normalize protein levels. All animals were then sacrificed 24 hours, 72 hours, or 288 hours (12 days) after the injection of EEDQ. D1 receptors and DRiP78 in the mPFC were estimated using a DRD1 ELISA kit and DRiP78 ELISA kit. Panel A: 24 hours after EEDQ administration, D1 receptor levels were substantially reduced, and no relationship was observed between D1 levels and animal’s general cognitive performance. Panel B: Partial recovery of D1 receptors was observed 72 hrs after EEDQ, and the the degree of recovery was related to animal’s GCA, r (18) = 0.57, p = 0.009, such that high GCA animals expressed a higher density of new D1 receptors. Panel C: By 288 hrs after EEDQ administration (when recovery of D1 receptors would have been complete), no relationship was observed between D1 levels and GCA. In total, this analysis indicates that under normal resting conditions, D1 levels are not related to GCA, while the rate of turnover of the D1 protein is positively related to GCA.