Fig. 2.

Tri-cistronic retroviral vectors function. a Schematic diagram of the CAR constructs. b Representative flow cytometry histograms of CAR, 4αβ chimeric cytokine receptor and hNIS expression on the cell surface of transduced T cells. c Fluorescence quantitation of cell surface expression of IL-4Rα. n = 4 donors/group. d Percentage cell viability of tumor cells 24 h after co-culture with T cells (1:1 E:T ratio) as determined by MTT assays. n = 4 donors/group. Graph presents mean ± s.e.m. **P < 0.01, ***P < 0.001. Supernatants from the experiments in c were used in ELISAs to determine the levels of (e) IFN-γ and TNF-α secretion by T cells. n = 4 donors/group. Graph presents mean ± s.e.m. ***P < 0.001. f Tumor burden of subcutaneous firefly luciferase-expressing PLP tumors treated with 4P28ζN or 4PTrN was assessed by BLI over a period of 21 days. n = 3 animals/group. Graph presents individual animal BLI signals