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. 2018 Mar 14;9:1081. doi: 10.1038/s41467-018-03524-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Establishing and validating hNIS function. a Relative in vitro uptake levels of T cells treated with ^99mTcO₄⁻ in the presence or absence of NaClO₄⁻. n = 3 donors/group. b Cell counts of untransduced or 4P28ζN T cells after 24 h culture in the presence or absence of ^99mTcO₄⁻. n = 4 donors/group. Graph presents mean ± s.e.m. c Relative in vitro uptake levels in live/dead untransduced or 4P28ζN T cells treated with ^99mTcO₄⁻. n = 4 donors/group. Graphs present mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001. d Relative T-cell ^99mTcO₄⁻ uptake over time. n = 6 donors/group. Graphs present mean + s.e.m. e Relative uptake of ^99mTcO₄⁻ by concentration. n = 6 donors/group. Graphs present mean + s.e.m. f Efflux of ^99mTcO₄⁻ over time shown as % of retained activity normalized to time 0. n = 3 donors/group. Graphs present mean + s.e.m.