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. 2018 Mar 14;8:4526. doi: 10.1038/s41598-018-22778-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hsc70-2 interacts with both BBSV p23 and CP. (a,b) BiFC analysis of the interactions between Hsc70-2 and p23 (a), and between Hsc70-2 and CP (b). Transient expression of mCherry-HDEL fusion was used to label the ER (ER-mCherry), and transgenic N. benthamiana expressing RFP-H2B (Histone H2B fused to RFP) was used to indicate the nucleus. NbrbcL indicates N. benthamiana RuBisCO large subunit (rbcL). White arrows point to the nuclei. Scale bars, 10 μm. (c,d) Co-IP analysis of the interactions between Hsc70-2 and p23 (c), and between Hsc70-2 and CP (d). (e,f) GST pull-down analysis of interactions between Hsc70-2 and p23^∆N52 proteins (e), and between Hsc70-2 and CP proteins (f).