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. 2018 Mar 14;8:4499. doi: 10.1038/s41598-018-22556-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of CuO NP treatment on murine lung inflammation. HE staining of the lung tissues harvested after 2.5, 5, and 10 mg/kg CuO NP treatment for 14 days in contrast to the saline control (a). qRT-PCR analysis of the major proinflammatory genes in the lung tissues after treatment with 5 mg/kg CuO NPs for 14 days (b). TGF-β levels in the BALF were measured using the ELISA kit. Representatives of at least 3 independent experiments are shown.