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. 2018 Mar 14;8:4536. doi: 10.1038/s41598-018-22692-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative abundance analyses at the OTU level and functional capacity profiles of the microbial communities of the ileum, cecum, and colon derived from 16S rRNA gene sequencing data. (A) Microbial composition at the phylum level. (B) Microbial composition at the genus level. Gut locations are represented along the horizontal axis, and relative abundance is denoted by the pie charts. (C) LEfSe identified significantly different OTUs according to the relative abundance among the three gut locations. The histogram shows the LDA scores computed for features at the OTU level, and each OTU was annotated at the lowest classification level (p: phylum, c: class, o: order, f: family, g: genus) according to the GreenGenes Database. OTUs in this graph were statistically significant (P < 0.05) and had an LDA Score >±4.0, which was considered a significant effect size. (D) Predicted function of the gut microbiota in the ileum, cecum, and colon. The vertical columns represent groups, and the horizontal rows depict metabolic pathways. The color coding is based on row z-scores.