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Sequences and affinities of single-chain Fvs with an identical heavy-chain complementarity-determining region 3 (HCDR3). Inverse PCR of the most abundant clone of the selected population was analyzed by Sanger sequencing. (A) Sanger sequence analysis of the eight unique clones with identical HCDR3 obtained by inverse PCR. (B) VDJ gene usage and affinity values of the eight different clones.
