Fig. 1.

OsKCH2 localizes to the PPB at prophase in vivo and transports AFs on the microtubule with minus-end-directed motility in vitro. a Schematic diagrams of the full-length OsKCH2 and OsKCH2(1–767). b OsKCH2 shows a punctate localization pattern along the PPB microtubules at prophase. Top and bottom rows are triple labeling of OsKCH2 (green), microtubules (red), and the nucleus (blue) in a rice cell at prophase when viewed from two different angles. c Coomassie-stained SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of purified recombinant OsKCH2(1–767). d Schematic diagram of the AF transport assay. e Micrograph montage showing that OsKCH2(1–767) transports rhodamine-labeled AFs (red) along an Alexa 488-labeled polarity-marked microtubule (green) toward the minus end. White arrowheads indicate the microtubule plus end, and red and yellow arrowheads indicate the leading ends of two different AFs. f Kymograph of two AFs shown in e moving at a fast velocity (V_fast) and a slow one (V_slow). g Velocity histogram of AF transport along microtubules with two distinct velocities. The velocity histogram was fitted to a combination of two Gaussian distributions. The green curve indicates the overall fit, and red and blue curves indicate the slow and fast velocity distribution, respectively. Scale bars: 1 min (vertical) and 5 µm (horizontal)