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. 2018 Mar 14;9:1067. doi: 10.1038/s41467-018-03480-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — CC2 enables GFP-OsKCH2(289-767) processivity by enhancing its microtubule-binding affinity. a Micrograph montage showing the gliding motion of polarity-marked microtubules (red) driven by surface-immobilized GFP-OsKCH2(289–720). White and brown arrowheads indicate the plus end of a polarity-marked microtubule at three different time points. b Example kymograph showing the nonprocessive motility of GFP-OsKCH2(289–720) on the microtubule. c Schematic diagrams of the full-length OsKCH2 and GST-OsKCH2(721–767). d Coomassie-stained SDS-PAGE of the microtubule co-sedimentation assay for GST-OsKCH2(721–767) in BRB80/25 mM KCl. e Coomassie-stained SDS-PAGE of the microtubule co-sedimentation assay for GST-OsKCH2(721–767) in BRB12. f Coomassie-stained SDS-PAGE of the microtubule co-sedimentation assay for GFP-OsKCH2(289–767) in BRB80/25 mM KCl. g Coomassie-stained SDS-PAGE of the microtubule co-sedimentation assay for GFP-OsKCH2(289–720) in BRB80/25 mM KCl. Scale bars: 30 s (vertical) and 5 µm (horizontal)