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. 2018 Mar 14;8:4520. doi: 10.1038/s41598-018-22129-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Average free energy profiles of promoters regions in different eukaryotes belonging to fungi, invertebrates (worm and fly) and vertebrates. The promoter sequences (−500 to +500 relative to translation start site (TLS)) are downloaded from SGD, CGD, and UCSC genome browser. The promoter sequences with GC percentage range 40–45 have been plotted. The red, green, blue, cyan, black and pink color plots represent fungi, worm, fly, marine invertebrate, fish and mammal including bird respectively. The model systems S. cerevisiae, C. elegans, D. melanogaster, zebrafish, mouse, and human are highlighted with gray background. The trends in AFE profiles are unique to closely related classes.