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. 2018 Mar 14;8:4520. doi: 10.1038/s41598-018-22129-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of ‘TP’ promoter predictions by PromPredict and EP3. Predictions in −1000 to +1000 region relative to TSS for both mouse and human have been considered. PromPredict uses a 250 nt sliding window with the midpoint of a 100 nt window being considered as a prediction, if it satisfies GC based cut-offs, while EP3 prediction is given in a 400 nt non-overlapping window and defines the window as a true or false prediction based on genome-specific thresholds. In case more than one ‘TP’ prediction is obtained for a gene, then the position closest to TSS has been considered. Hence, the total number of TPs plotted corresponds to ‘TP genes’.