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. 2018 Mar 14;9:1086. doi: 10.1038/s41467-018-03272-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — D2R upregulation reduces D2-MSN inhibitory transmission to ventral pallidum (VP). a Schematic of oIPSCs recordings from VP neurons in slice following blue-light stimulation of terminal fields from NAc MSNs co-expressing ChR2 and D2R or ChR2 and EGFP. b VP neurons were recorded from within virus-positive NAc D2-MSN terminal fields in the dorsolateral VP (dlVP). Scale bar = 150 µm. c Target neurons in VP typically displayed spontaneous firing activity, depolarized RMP, slow ramp-like depolarization preceding short duration spikes, and prominent afterhyperpolarization. d Individual (gray) and average (black) oIPSC traces (gray) from representative MSNs following five 50-ms light pulses (1 Hz). Bicuculline (10 µM) eliminated the oIPSC. e Peak oIPSC amplitude under basal conditions was significantly reduced when D2Rs were overexpressed in D2-MSNs MSNs [n = 13 cells (10 mice) or 11 cells (7 mice)]. f Quinpirole (1 µM) bidirectionally modulated oIPSCs recorded in EGFP_NAcInd VP neurons MSNs [n = 11 cells (9 mice) or 10 cells (6 mice)]. In contrast, quinpirole decreased the oIPSC amplitude in >90% of cells recorded in D2R-OE_NAcInd VP. g Opposite influences of D2 agonist and antagonist are highlighted by the overall effect of quinpirole and quinpirole + sulpiride (1 µM) on oIPSC amplitude. Error bars = s.e.m.