Figure 4.

Schematic diagram of one possible multiplexing scheme envisioned for functionalized xenon biosensors. The top spectrum shows the three distinct functionalized xenon peaks, corresponding to different cages linked to three ligands. The bottom spectrum shows the effect of adding the functionalized xenon to an unknown solution. On addition to the unknown solution, the leftmost peak shifts entirely, representing the case in which all functionalized xenon is bound to its corresponding protein. The central peak decreases in intensity, and a peak corresponding to the protein-bound functionalized xenon appears. The rightmost peak remains unaffected, indicating the absence of the corresponding protein target. The color of each xenon atom reflects its chemical shift in accordance with the color bar shown beneath the spectra.