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. 2018 Mar 8;9:385. doi: 10.3389/fmicb.2018.00385

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Copyright © 2018 Kumar, Kumar, Das, Kumar, Abhishek, Verma, Mandal, Singh and Das.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of miR-210 and HIF-1α in infected macrophages. Four different experimental conditions (Mφ, Mφ+LD, siHIF-1α+Mφ+LD, and scHIF-1α+Mφ+LD) were used for expression analysis of HIF-1α by semi-quantitative PCR (A). The expression of miR-210 in L. donovani infected macrophages in the presence of siHIF-1α or scHIF-1α was estimated by qPCR (B). The sno-202 was taken as endogenous control. The application of siHIF-1α decreased (p < 0.001) the level of miR-210 expression in infected macrophages (B).