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. 2018 Mar 8;9:282. doi: 10.3389/fpls.2018.00282

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Copyright © 2018 He, Gao, Dou, Shao, Bi, Sheng, Deng, Li, Hu, Liu, Zhang, Yang and Yi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of log₂ iTRAQ ratio (115/114 and 116/114) for 1,834 proteins identified in all three Dajiao biological replicates: set1, set2, and set3. Plots of 115/114 or 116/114 ratios for each of the quantified proteins between two of the three sets generated comparable quantification results, as determined by a linear regression analysis that revealed a slope of around 0.96, 0.97, or 0.99 in Dajiao for 115/114, among the pairs of set1/set2 (A), set1/set3 (B), and set2/set3 (C), respectively. Similar plots were also conducted for the 116/114 ratio, yielding a slope of around 0.96, 0.96, or 0.97 in Dajiao, among the pairs of set1/set2 (D), set1/set3 (E), and set2/set3 (F), respectively.