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. 2018 Mar 14;9(3):398. doi: 10.1038/s41419-018-0428-x

Fig. 5. HDAC3 specifically interacts with STAT3 in the cytoplasm.

Fig. 5

a, b Western blot analysis demonstrates that the level of ac-STAT3 is high in the HDAC3△ liver at 6 h after PH. Histone H3 was used as the loading control. c Immunoprecipitation shows that HDAC1, HDAC2, and HDAC3 can each combine with ac-STAT3 in the nucleus following PH. d Immunoblotting to determination of the cytoplasmic and nuclear distribution shows that ac-STAT3 is primarily localized to the cytoplasm at 6 h after PH. β-Actin and histone H3 were used as the loading controls. e Immunoblotting to determination of the cytoplasmic and nuclear distributions of HDAC1, HDAC2, and HDAC3 in the control and HDAC3△ mice demonstrates that HDAC3 localizes to both the cytoplasm and nucleus, whereas HDAC1 and HDAC2 strictly localize to the nucleus. f Co-immunoprecipitation assays demonstrate that HDAC3, but not HDAC1 or HDAC2, combines with STAT3 in the cytoplasm of hepatocytes from control livers. All data represent the mean ± SD; n = 3–5; ***p < 0.001