Fig. 6. HDAC3 is highly expressed in HCC subtypes and is correlated with a poor prognosis in HCC patients.

a Representative microphotograph and statistical analysis of HDAC3 and p-STAT3(Y705) expression in HCC tissues (n = 90) by immunohistochemistry (scale bar: 50 μm). b Co-immunoprecipitation shows the combination between STAT3 and HDAC3 or p300 in HDAC3-positive (+) or HDAC3-negative (−) HCC tissues. c Immunoblotting demonstrates that p-STAT3(Y705) increases in HDAC3-positive HCC tissues. NL normal liver, T tumor tissue, NT adjacent non-tumor tissues. GAPDH was used as the loading control. d Kaplan–Meier analysis shows that single upregulation of HDAC3 (n = 90) or combined upregulation of HDAC3 and p-STAT3(Y705) (n = 42) significantly reduces the overall survival rate of HCC patients. e Analysis of TCGA data revealed that HCC patients with high HDAC3 expression (Z score >1) were significantly associated with a poorer overall survival. FPKM fragments per kilobase of exon per million fragments mapped. All data represent the mean ± SD; ***p < 0.001