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. 2018 Mar 14;8:4556. doi: 10.1038/s41598-018-22598-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Processes of seeding and culturing cells on the microplates. (a) The glass substrate with microplates was placed in a petri dish. (b) NIH/3T3 cells were seeded on the microplates, and non-adherent cells were washed away. (c) Adherent NIH/3T3 cells were cultured for 24 h. (d) HepG2 cells were then seeded onto plates and non-adherent cells were washed away. (e) The attached HepG2 cells were cultured 4 h on the NIH/3T3 cells which loaded on the microplates. (e,f) After adding alginate lyase, the microplates were folded, and a number of 3D cell co-culture microstructures were formed.