Fig. 5. Nkx2.5^Cre/+;Bmp2^tg/+ embryos show defective ventricular chamber maturation.

a E14.5, ISH. The black arrowheads mark compact myocardium, arrows mark trabeculae, white arrowheads the AVC valves region and the asterisks indicate a ventricular septal defect. ISH of Hey2, n-Myc, Tbx20 and Tbx2 in E14.5 WT and Nkx2.5^Cre/+;Bmp2^tg/+ hearts. b ISH of Bmp10, Cx40, Cx43, Anf, Hand1, Sema3a, Smad6, Gata4 and Id2 in E14.5 WT and transgenic hearts. IVS interventricular septum, rv right ventricle, lv left ventricle. Scale bars 200 μm. c Left, Circular plot of 42 differentially expressed genes in E14.5 transgenic hearts. Top, Chart showing the total number of upregulated (red) and downregulated genes (green). Details are available in Supplementary file 1. d B-H P value, Benjamini–Hochberg-adjusted P value. % changed classifies the genes altered in Nkx2.5^Cre/+;Bmp2^tg/+ transgenic hearts as a percentage of the total number of genes involved in the listed biological function. t test *P < 0.05; **P < 0.01; ***P < 0.005