Table 3.

Comparison of total signal difference between LCs and solutions in the presence of buffer and at different ionic strengths and magnesium and sodium concentrations to signals obtained in H₂O alone for LC1 and S1 or in buffer for LC2 and S2, respectively.

Liquid crystal/liquid crystal	Total signal differences	Solution/solution	Total signal differences
LC2/LC1	45.04	S2/S1	60.72
LC3/LC2	0.95	S3/S2	13.43
LC4/LC2	26.88	S4/S2	13.46
LC5/LC2	15.89	S5/S2	11.91
LC6/LC2	6.89	S6/S2	11.11

The total signal difference was calculated as the sum of log₂(IntLC/IntLC) or log₂(IntS/IntS) values at all Y-Y positions.