Fig. 2.

Cell proliferation but not pluripotency marker expression is altered in Nsun3 ^cat/cat ESCs. a Wild-type and Nsun3-mutant cells were seeded into 96-well plates and cell numbers were determined using the CyQuant^® fluorescence assay at the indicated time points. Mean ± SEM of five technical replicates are shown. Statistically significant differences were found for 72 h (**p = 0.007) and 96 h (***p = 0.0004) values by unpaired t test. b RT-qPCR was performed to determine the expression levels of pluripotency markers in Nsun3 ^cat/cat relative to wild-type cells. Mean ± SEM of three experiments are shown. Statistical significance was determined by multiple unpaired t test with Holm–Sidak correction (*p < 0.05)