Fig. 1.

SBDβ mutations alter thermotolerance and [PSI ⁺] propagation. a Locations of SBD mutants on the DnaK structure. Ribbon schematic representations of the Protein Data Bank structure 1Q5L. The DnaK amino acid residue numbers are homologous to those of the Ssa1 mutations assessed. b Assessment of [PSI ⁺] propagation. [PSI ⁺] propagation assay at RT. Single colonies were streaked on YPD and −ADE plates which were then incubated at RT for 5–7 days. [psi ⁻] cells were red colonies on YPD and unable to grow on−ADE plates; [PSI ⁺] cells were white colonies on YPD and viable on −ADE plates. c Growth assay of SBD mutants at elevated temperatures. Fresh cultures were spotted onto YPD after a 1/5 serial dilution. Plates were incubated at 30 °C for 2 days