Figure 8.

Co-localization of TRPA1 channel and HSP47 in mouse intestine. (A) Vehicle, (B) TNBS: Immunostaining images of mouse intestine stained with anti-TRPA1 (red) antibody, anti-HSP47 (green), and 4′,6-diamidino-2-phenylindole (DAPI) (blue). (C) TRPA1 mRNA expression in vehicle and TNBS-treated colitis mouse (n = 5). (D) Number of TRPA1/HSP47 double-positive cells per unit area: 10 mm³ counted from immune-staining data (n = 5). (E) Western blot analysis of TRPA1 and β-actin in WT vehicle and WT TNBS mouse distal colon (n = 5). *P < .05 vs vehicle.