Table 2.
Quantitative Reverse-Transcription PCR Primer Sequences (5’ to 3’)
| Forward primer | Reverse primer | |
|---|---|---|
| Tfr1 | GAGGCAGACCTTGCACTCTT | TGACTGAGATGGCGGAAAC |
| Fpn1 | GCCACTGCGATCACAATCC | TGGAGTTCTGCACACCATTGAT |
| Hamp1 | TTGCGATACCAATGCAGAAG | GGATGTGGCTCTAGGCTATGTT |
| Adiponectin | GGAGATGCAGGTCTTCTTGG | TCCAGGCTCTCCTTTCCTG |
| Leptin | GCAGTGCCTATCCAGAAAGTCC | GGAATGAAGTCCAAGCCAGTGAC |
| Resistin | CATGCCACTGTGTCCCATCGAT | ACTTCCCTCTGGAGGAGACTGT |
| Rbp-4 | TGTAGCCTCCTTTCTCCAGCGA | ACAGGTGCCATCCAGATTCTGC |
| B2-mg | CTGATACATACGCCTGCAGAGTTAA | ATGAATCTTCAGAGCATCATGAT |
| Btf-3 | TGGCAGCAAACACCTTCACC | AGCTTCAGCCAGTCTCCTTAAAC |
| Gapdh | TCCTGCACCACCAACTGCTTAGC | GCCTGCTTCACCACCTTCTTGAT |
| Polr2a | AGCTGGTCCTTCGAATCCGC | CTGATCTGCTCGATACCCTGC |
| β-actin | CATTGCTGACAGGATGCAGAAGG | TGCTGGAAGGTGGACAGTGAGG |
| Hprt | GGACTGATTATGGACAGGA | GAGGGCCACAATGTGATG |
| Hmox1 | CACTCTGGAGATGACACCTGAG | GTGTTCCTCTGTCAGCATCACC |
Btf3, basic transcription factor-3 gene; β2-mg, β2-microglobulin gene; Gapdh, glyceraldehyde-3-phosphate dehydrogenase gene; Hmox1, heme oxygenase 1; Hprt, hypoxanthine guanine phosphoribosyl transferase gene; Polr2a, RNA polymerase II subunit A gene.