Figure 2.

The miR‐634 gene was highly methylated in gastric cancer cell lines and cancer tissues. (A) Quantitative real‐time PCR (qRT‐PCR) was used to detect the expression of the miR‐634 gene in gastric cancer (GC) cell lines treated or untreated with 5‐aza‐2 ‐deoxycytidine (5‐aza‐d C). (B) The methylation‐specific PCR (MSP) method was used to detect the methylation status of the miR‐634 gene in gastric cancer cell lines treated or untreated with 5‐aza‐d C. −, 5‐aza‐d C untreated; +, 5‐aza‐d C treated. (C and D) The relationships between methylation status and expression of miR‐634 in GC tumor tissues. *, P < 0.05