Fig. 5.

Intrinsic disorder in linkers of distant homology. Top: Primary sequences of the Apibacter mensalis (Am) and Gelidibacter mesophilus (Gm) CFBPs were aligned by Clustal Omega, and the three regions (CYN, loop, FKBP) are indicated by color (Teal, Dark Blue, and Red, respectively); the linker is additionally in bold. Asterisks and dots respectively indicate identical and conservatively replaced amino acids. Note the significantly lower amino acid identity in the linker region, but higher conservative replacements, contrasting with the high amino acid identity in the CYN and FKBP regions. Bottom: Disorder graph for the two single CFBPs as named, plotted as in Fig. 4. Note that the highest regions of disorder coincide with the respective linker positions.