Figure 1.

Immunohistochemistry. (A) Representative CD8 stain after annotation by CD3 Quantifier image analysis. Stromal areas have been manually encircled (red) and were not evaluated. (a) Stained lymphocytes are labeled by a thin blue line; (b) same picture as in a, with annotated lymphocytes shown as blue areas. (B) MHC1 expression in HGSOC. (a) Example of a tumor with low, focal expression; (b) example of a tumor with strong diffuse expression. A membranous and cytoplasmic expression pattern is evident. (C) MHC2 expression in HGSOC. (a) Weak and focal expression; single cells with strong expression are intratumoral immune cells (which were not evaluated; arrows); (b) tumor with diffuse expression with varying intensity revealing a mosaic-like pattern; expression is mainly cytoplasmic in these examples.